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Confirmation is accomplished by comparison of ion ratios with those of a pure synthesized standard of clenbuterol oxazolidin-3-one obtained by selected ion monitoring, electron ionization gas chromatography/mass spectrometry on a benchtop instrument. The detection of clenbuterol in medical research, veterinary and anti-doping fields is conducted by a variety of methods, including immunoassays ( 5 ), capillary electrophoresis assays ( 6) and chromatographic–spectrometric techniques ( 7 ). Clenbuterol concentrations were determined in plasma and urine by liquid chromatography-mass spectrometry (LC-MS/MS) using a stock solution of clenbuterol-d9 as internal standard to verify quantitative accuracy and precision. For comparative analysis of clenbuterol in plasma and liver samples, both GC-MS and enzyme immunoassay (EIA) methods are found to be suitable. Due to potential antibody-cross reactivity with EIA, the GC-MS method is the method of choice for most samples because of its specificity. Sample preparation procedures and analytical techniques used for the detection of clenbuterol are manifold and vary with the objectives of the investigation. Methods for its detection in biological specimens, drug preparations, the environment, food and feed products are reported. This profile also includes the monograph of British Pharmacopoeia, together with several reported analytical methods including spectrophotometric, electrochemical, chromatographic, immunochemical methods, and capillary electrophoretic methods. The stability, the pharmacokinetic behavior, and the pharmacology of the drug are also provided
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